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ep300 overexpression lentiviral vectors  (Genechem)

 
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    Genechem ep300 overexpression lentiviral vectors
    Ep300 Overexpression Lentiviral Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ep300+overexpression+lentiviral+vectors/ep300+lentiviral+overexpression+vectors/pm41946791-82-0-11
    Average 86 stars, based on 1 article reviews
    ep300 overexpression lentiviral vectors - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Over Expression:

    Article Title: Curcumin inhibits glycolysis via EP300 in oral squamous cell carcinoma
    Article Snippet: .. EP300 overexpression lentiviral vectors and corresponding empty vectors were purchased from Genechem (China), which contained puromycin (Solarbio, China) resistance sequences.UM-SCC-1 cells at 70–80% confluence were transfected using the corresponding transfection reagent according to the manufacturer’s instructions. ..

    Article Title: Curcumin inhibits glycolysis via EP300 in oral squamous cell carcinoma.
    Article Snippet: .. EP300 overexpression lentiviral vectors and corresponding empty vectors were purchased from Genechem (China), which contained puromycin (Solarbio, China) resistance sequences.UM-SCC-1 cells at 70–80% confluence were transfected using the corresponding transfection reagent according to the manufacturer’s instructions. ..

    Transfection:

    Article Title: Curcumin inhibits glycolysis via EP300 in oral squamous cell carcinoma
    Article Snippet: .. EP300 overexpression lentiviral vectors and corresponding empty vectors were purchased from Genechem (China), which contained puromycin (Solarbio, China) resistance sequences.UM-SCC-1 cells at 70–80% confluence were transfected using the corresponding transfection reagent according to the manufacturer’s instructions. ..

    Article Title: Curcumin inhibits glycolysis via EP300 in oral squamous cell carcinoma.
    Article Snippet: .. EP300 overexpression lentiviral vectors and corresponding empty vectors were purchased from Genechem (China), which contained puromycin (Solarbio, China) resistance sequences.UM-SCC-1 cells at 70–80% confluence were transfected using the corresponding transfection reagent according to the manufacturer’s instructions. ..



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    ep300 overexpression lentiviral vectors  (Genechem)
    86
    Genechem ep300 overexpression lentiviral vectors
    Ep300 Overexpression Lentiviral Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ep300+overexpression+lentiviral+vectors/ep300+lentiviral+overexpression+vectors/pm41946791-82-0-11
    Average 86 stars, based on 1 article reviews
    ep300 overexpression lentiviral vectors - by Bioz Stars, 2026-11
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    		  Buy from Supplier
    ep300 overexpression lentiviral vector  (Genechem)
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    Genechem ep300 overexpression lentiviral vector
    HDAC6 induces extensive chromatin modifications at FOXP3 loci. A. Histone acetyltransferase (HATs) and histone deacetylases (HDACs) affect the dynamics of chromatin remodeling during gene transcription. B. Schematic diagram depicting FOXP3 locus structure and the sequences in the FOXP3 promoter and CNS regions. C. ChIP assays for H3Ac, H3K9Ac, H3K27Ac, H4Ac modifications in FOXP3 locus. IgG serves as immunoprecipitation control. Student’s t-test. D. ChIP assays for HDAC6 in FOXP3 promoter and CNS regions in GES-1 cells. Student’s t-test. E. FOXP3 and HNF4α protein levels was examined by western blot analysis in AGS cells treated with <t>EP300-expressing</t> vectors or control. *P < 0.05, **P < 0.01. N.S., not significant.
    Ep300 Overexpression Lentiviral Vector, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hdac6, foxp3, hnf4α ep300 overexpression lentiviral vectors  (Genechem)
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    Genechem hdac6, foxp3, hnf4α ep300 overexpression lentiviral vectors
    Sequence of primers used in the study
    Hdac6, Foxp3, Hnf4α Ep300 Overexpression Lentiviral Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HDAC6 induces extensive chromatin modifications at FOXP3 loci. A. Histone acetyltransferase (HATs) and histone deacetylases (HDACs) affect the dynamics of chromatin remodeling during gene transcription. B. Schematic diagram depicting FOXP3 locus structure and the sequences in the FOXP3 promoter and CNS regions. C. ChIP assays for H3Ac, H3K9Ac, H3K27Ac, H4Ac modifications in FOXP3 locus. IgG serves as immunoprecipitation control. Student’s t-test. D. ChIP assays for HDAC6 in FOXP3 promoter and CNS regions in GES-1 cells. Student’s t-test. E. FOXP3 and HNF4α protein levels was examined by western blot analysis in AGS cells treated with EP300-expressing vectors or control. *P < 0.05, **P < 0.01. N.S., not significant.

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: HDAC6 induces extensive chromatin modifications at FOXP3 loci. A. Histone acetyltransferase (HATs) and histone deacetylases (HDACs) affect the dynamics of chromatin remodeling during gene transcription. B. Schematic diagram depicting FOXP3 locus structure and the sequences in the FOXP3 promoter and CNS regions. C. ChIP assays for H3Ac, H3K9Ac, H3K27Ac, H4Ac modifications in FOXP3 locus. IgG serves as immunoprecipitation control. Student’s t-test. D. ChIP assays for HDAC6 in FOXP3 promoter and CNS regions in GES-1 cells. Student’s t-test. E. FOXP3 and HNF4α protein levels was examined by western blot analysis in AGS cells treated with EP300-expressing vectors or control. *P < 0.05, **P < 0.01. N.S., not significant.

    Article Snippet: HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Immunoprecipitation, Western Blot, Expressing

    Sequence of primers used in the study

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: Sequence of primers used in the study

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Sequencing

    Sequence of primers used in the study

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: Sequence of primers used in the study

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Sequencing

    FOXP3 was decreased in gastric intestinal metaplasia (IM). A. qRT-PCR results of expression of FOXP3 in 10 paired IM and surrounding tissues. B. Comparison of FOXP3, HDAC6 and HNF4α mRNA expression in the biopsies. **P < 0.01.

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: FOXP3 was decreased in gastric intestinal metaplasia (IM). A. qRT-PCR results of expression of FOXP3 in 10 paired IM and surrounding tissues. B. Comparison of FOXP3, HDAC6 and HNF4α mRNA expression in the biopsies. **P < 0.01.

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Quantitative RT-PCR, Expressing

    HDAC6 induces extensive chromatin modifications at FOXP3 loci. A. Histone acetyltransferase (HATs) and histone deacetylases (HDACs) affect the dynamics of chromatin remodeling during gene transcription. B. Schematic diagram depicting FOXP3 locus structure and the sequences in the FOXP3 promoter and CNS regions. C. ChIP assays for H3Ac, H3K9Ac, H3K27Ac, H4Ac modifications in FOXP3 locus. IgG serves as immunoprecipitation control. Student’s t-test. D. ChIP assays for HDAC6 in FOXP3 promoter and CNS regions in GES-1 cells. Student’s t-test. E. FOXP3 and HNF4α protein levels was examined by western blot analysis in AGS cells treated with EP300-expressing vectors or control. *P < 0.05, **P < 0.01. N.S., not significant.

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: HDAC6 induces extensive chromatin modifications at FOXP3 loci. A. Histone acetyltransferase (HATs) and histone deacetylases (HDACs) affect the dynamics of chromatin remodeling during gene transcription. B. Schematic diagram depicting FOXP3 locus structure and the sequences in the FOXP3 promoter and CNS regions. C. ChIP assays for H3Ac, H3K9Ac, H3K27Ac, H4Ac modifications in FOXP3 locus. IgG serves as immunoprecipitation control. Student’s t-test. D. ChIP assays for HDAC6 in FOXP3 promoter and CNS regions in GES-1 cells. Student’s t-test. E. FOXP3 and HNF4α protein levels was examined by western blot analysis in AGS cells treated with EP300-expressing vectors or control. *P < 0.05, **P < 0.01. N.S., not significant.

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Immunoprecipitation, Western Blot, Expressing

    FOXP3 negatively regulated HNF4α transcription. A. FOXP3 inversely regulated HNF4α at both mRNA and protein levels. Student’s t-test. B. The promoter activity of HNF4α was enhanced by DCA and siFOXP3 treatment in GES-1 cells. Student’s t-test. C. A ChIP assay demonstrated the direct binding of FOXP3 to the HNF4α promoter in GES-1 cells. Student’s t-test. D. AGS cells were co-infected with FOXP3-expressing vectors and HNF4α-expressing vectors. The expression of intestinal markers was detected by immunoblot. E. Upregulated intestinal markers expression induced by siFOXP3 was attenuated by siHNF4α in GES-1 cells. F. A schematic model of miR-1/HDAC6/FOXP3/HNF4α pathway in gastric cells. In response to BA, miR-1 inhibition promotes HNF4α and HDAC6, which increase the transcription of intestinal markers including MUC2, KLF4 and CDX2. **P < 0.01. N.S., not significant.

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: FOXP3 negatively regulated HNF4α transcription. A. FOXP3 inversely regulated HNF4α at both mRNA and protein levels. Student’s t-test. B. The promoter activity of HNF4α was enhanced by DCA and siFOXP3 treatment in GES-1 cells. Student’s t-test. C. A ChIP assay demonstrated the direct binding of FOXP3 to the HNF4α promoter in GES-1 cells. Student’s t-test. D. AGS cells were co-infected with FOXP3-expressing vectors and HNF4α-expressing vectors. The expression of intestinal markers was detected by immunoblot. E. Upregulated intestinal markers expression induced by siFOXP3 was attenuated by siHNF4α in GES-1 cells. F. A schematic model of miR-1/HDAC6/FOXP3/HNF4α pathway in gastric cells. In response to BA, miR-1 inhibition promotes HNF4α and HDAC6, which increase the transcription of intestinal markers including MUC2, KLF4 and CDX2. **P < 0.01. N.S., not significant.

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Activity Assay, Binding Assay, Infection, Expressing, Western Blot, Inhibition

    HDAC6/FOXP3/HNF4α loop in mice. A. Experimental strategy of establishing gastric intestinal metaplasia model in mice. Active Hnf4α expression was induced after tamoxifen treatment. B. Representative pictures of IHC staining for FOXP3, HDAC6 and HNF4α at the SCJ of WT and Rosa26Hnf4α mice with or without DCA treatment. Scale bars: 20 µm (bottom). C. Expression of these molecules in the gastric antrum of four groups of mice. Scale bars: 20 µm (bottom).

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: HDAC6/FOXP3/HNF4α loop in mice. A. Experimental strategy of establishing gastric intestinal metaplasia model in mice. Active Hnf4α expression was induced after tamoxifen treatment. B. Representative pictures of IHC staining for FOXP3, HDAC6 and HNF4α at the SCJ of WT and Rosa26Hnf4α mice with or without DCA treatment. Scale bars: 20 µm (bottom). C. Expression of these molecules in the gastric antrum of four groups of mice. Scale bars: 20 µm (bottom).

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: Expressing, Immunohistochemistry

    Histopathological changes of gastric mucosa in mice (χ 2 test)

    Journal: American Journal of Cancer Research

    Article Title: HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia

    doi:

    Figure Lengend Snippet: Histopathological changes of gastric mucosa in mice (χ 2 test)

    Article Snippet: Transfection and infection HDAC6, FOXP3, HNF4α and EP300 overexpression lentiviral vectors were purchased from Genechem Co. Ltd. (China).

    Techniques: